Olympus Live
Location: T-lab level 9, C.elegans room
Contact Persons: Mak Kah Jun/Dr Peng Qiwen
Heater for micoscope chamber
The heater should be left ON at all times
CO2 controller
Switch on the CO2 controller if necessary.
Humidifier
Fill the humidifier with MQ water
CO2 cover
Use the on-stage incubator with CO2 supply and humidity control from the water bottle for live cell imaging.
Main tab
Select the relevant options
Saving tab
Select your filename and save location
Do not save in C: drive, system might crash during your experiment.
Backup and delete your data from imaging system as soon as possible, other users might delete your data if the system runs out of space.
Timelapse experiment tab
Setup your timelapse intervals here
Multi-position experiment tab
Setup your various positions by clicking on the "+" sign, positions can be preloaded with a file format that is similar to CSV
Multi-fluorescence experiment tab
Setup by keying in the total number of different fluoroscence acquisitions needed
Fluorescence/camera settings sub tab
Setup the camera exposure time and frequency of acquisition
Z-stack experiment tab
Setup the z-stack by either setting top and bottom positions or using current position as the mid point, followed by step size and number of steps
Summary tab
Read through the summary for a final check
