MBI | W1 LiveSR (L10) Spinning Disk
Location: T-lab level 10, room 4
Contact Persons: Mak Kah Jun/Dr Chin Feili, Jasmine
Turn the key to on to enable the lasers
Switch on the individual lasers that are needed for your experiment
488nm laser controller
Turn the key from standby to on a few seconds after switching it on.
If laser ready light does not appear or turn green, turn the key to standby and back to on again.
Heater for micoscope chamber
Switch on the wall plug for the heater
Switch on the fluorescent light source if necessary
Switch on the CO2 controller if necessary.
Fill the humidifier with MQ water
Select the relevant options
Select your filename and save location
Do not save in C: drive, system might crash during your experiment.
Backup and delete your data from imaging system as soon as possible, other users might delete your data if the system runs out of space.
Timelapse experiment tab
Setup your timelapse intervals here
Multi-position experiment tab
Setup your various positions by clicking on the "+" sign, positions can be preloaded with a file format that is similar to CSV
Multi-fluorescence experiment tab
Setup by keying in the total number of different fluoroscence acquisitions needed
Fluorescence/camera settings sub tab
Setup the camera exposure time and frequency of acquisition
Z-stack experiment tab
Setup the z-stack by either setting top and bottom positions or using current position as the mid point, followed by step size and number of steps
Read through the summary for a final check