Location: T-lab level 9, room 7
Contact Persons: Mak Kah Jun/Liu Jun
Turn the relevant plugs to the on position, all plugs are labelled with the attached component's name
If 488nm laser is needed, ensure the switch is flipped to the on position before turning the key to start the laser
If 561nm laser is needed, ensure that the screen shows that it has finished booting up before turning the key to start the laser.
405nm & 640nm lasers
If 405nm laser is needed, turn the key to start the laser
If 640nm laser is needed, ensure the switch is flipped to the on position before turning the key to start the laser
If incubation is needed, press and hold on the power button to switch on the stage top incubation heating elements
Individual ND filters are available to attenuate the laser power.
If laser power seems to be insufficient, check to see that these ND filters are not engaged.
Software selection mode
For easy STORM acquisition, select the ANDOR with N-STORM mode
For other studies or for advanced processing, select the ANDOR alone mode
General interface of the software
To get missing control panels which have been closed by the previous user, right click, go to Acquisition Controls, select the relevant control panel
List of system presets, can be overwritten by clicking the small left facing arrow button besides each setting
DU-897 Settings/Camera settings
Exposure, Binning and EM Gain settings
Important: Keep EM Gain below 300 unless sample is very dim and image does not have saturated pixels
Various adjustments for the microscope
Red arrow points to the TIRF angle adjustment
Release interlock for control of the lasers, which is available only when the light path is directed to the camera
Simplified N-STORM acquisition panel with option for direct "Live" STORM image and graphing option to show pixel counts per frame for experiment optimisation.
For more options in acquisition
N3 Laser License Required to operate the microscope