Cyntellect LEAP



Magnification of the system: 3x, 5x, 10x, 20x

Excitation (nm)
Dichroic (nm)
Emission (nm)
Common Dyes
 377/50 409 LP
 438/24 DAPI, Hoechst, Alexa Fluor 350, Aminomethylcoumarin, Lysosensor Blue, Marina Blue, Pacific Blue, BFP, EBFP
 438/24 458 LP
 483/32AmCyan, CFP, ECFP, BOBO-1, SYTOX Blue
 472/30 495 LP
 520/35Cy2, CyQuant, DiO, YOYO-1, GFP, EGFP,
 482/35 506 LP
 536/40Carboxyfluorescein. FITC, Alexa Fluor 488, Calcein, Fluo-4, Mitotracker Green, Oregon Green, Rhodamine 110, rsGFP
 525/40 555 LP
 585/40Rhodamine, TMR, TRITC, Alexa Fluor 532, 5-TAMRA, Dil, POPO-3, PO-PRO-3,
 531/40 562 LP
 593/40Alexa Fluor 546, Alexa Fluor 555, Calcium Orange, Cy3, LysoTracker Yellow, MitoTracker Orange, Phycoerythrin (PE), dsRed
 562/40 593 LP
 624/40Propidium Iodide (PI), Cy3.5, Alexa Fluor 568, Alexa Fluor 594, Calcium Crimson, MitoTracker Red, Texas Red, HcRed
 628/40 660 LP
 692/25Cy5, Alexa Fluor 647, Alexa Fluor 660, APC (Allophycocyanin), BODIPY 650/665, DiD, SYTO Red 60, 62, 63, TOTO-3

Do note that due to the flexible combination of the filters, special dyes such as QDots are not an issue. Please check with us if you have any queries







SOP

Start-up
1. Turn on the LEAP instrument by switching on the main power button.
2. Turn on the LEAP computer.
3. Launch the LEAP application using the “LEAP” icon, located on the desktop.
The program opens and the initial startup screen, called Power System
Warm-Up Status
4. Tick on the Excitation Lamp and wait for it to be warmed up.

Pre-operation
1. Select magnification
2. Select the Laser to be used
3. Set the Laser power and spot size (with guidance from the Excel Spreadsheet on the desktop)


Operation
1. Load the plate
2. Select the plate format
3. Load the Cell Purification Protocol
4. Load the channel settings (Example: channel 1 DAPI, Channel 2 GFP)
5. Optimise the exposure time and segmentation settings
6. Select the wells to be used
7. Run the protocol
8. Process the well for the Laser to fire
9. Run protocol again to verify selection efficiency

Shut-down
1. Remove your plate
2. Close the LEAP software
3. Shutdown the computer
4. Power off the main system


Sample preparation

Please read attached phototherm reagent file
Ċ
Kah Jun Mak,
Aug 17, 2011, 12:00 AM
Ċ
Kah Jun Mak,
Aug 17, 2011, 12:00 AM
Ċ
Kah Jun Mak,
Aug 17, 2011, 12:00 AM
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